Proteomic analysis for the cell-death related prteins makes it possible to address the pathophysiology of sepis

2015 Fiscal Year Final Research Report

• Project/Area Number: 25462831
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Emergency medicine
• Research Institution: Juntendo University
• Principal Investigator: Iba Toshiaki 順天堂大学, 医学部, 教授 (40193635)
• Co-Investigator(Kenkyū-buntansha): HAYASHI Nobuhiro 東京工業大学, 生命理工学研究科, 准教授 (80267955)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: proteome / sepsis / cell-death / DAMPs / histone / nucleosome / protein C / pentraxin 3

Outline of Final Research Achievements

It is known that various types of cell-death is induced in sepsis. Damage-associated molecular patterns from the dead cell is know to activate further inflammation. In the first study, we measured circulating levels of DAMPs in the sepsis patient. The result shows that the levels of histones, nucleosome and HMGB-1 increases significantly in non-survivors. Thus, we think these DAMPs are useful as bio-markers for severity of sepsis. In the second study, we examined the toxicity of DAMPs to the vascular endothelial cells. The results showed that histone H3 and H4 showed the strong toxicity. In the third study, we examined the protective effects of physiological serum proteins. As a result, significant protective effects of either albumin, activated protein C or pentraxin 3 to the histone H3 were revealed.

Free Research Field

救急医学