Elucidation of the role of Early growth response factor (Egr)-1 in renal ischemia reperfusion injury in mice

2015 Fiscal Year Final Research Report

• Project/Area Number: 25462836
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Emergency medicine
• Research Institution: Nippon Medical School
• Principal Investigator: Arai Masatoku 日本医科大学, 医学部, 助教 (60267127)
• Co-Investigator(Kenkyū-buntansha): TSUKAMOTO Takeshi 日本医科大学, 医学部, 助教 (20626270) ; MASUNO Tomohiko 日本医科大学, 医学部, 講師 (00318528) ; YOKOTA Hiroyuki 日本医科大学, 医学部, 教授 (60182698)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 虚血再灌流障害 / 腎障害 / 腫瘍壊死因子 / Egr-1

Outline of Final Research Achievements

We examined the role of early growth response factor-1 (Egr-1) in renal ischemia-reperfusion (I/R) injury in mice. Serum levels of BUN and creatinine, and histologic changes of kidneys were significantly increased at 12 ~48 hours after renal I/R. Renal tissue levels of TNF and MPO activity were significantly increased at 3 and 6 hours after renal I/R respectively. The expression of Egr-1 by Western blot analysis, significantly increased at 60~90 min after renal I/R and decreasing thereafter. These observations suggest that the increase of Egr-1 expression may be involved with renal I/R injury through the production of TNF and accumulation of neutrophils in mice.

Free Research Field

救急医学