Study on colonization mechanism of periodontal pathogens: preparation of infection experiment under anaerobic conditions and analysis of fimbrial functions for colonization

2015 Fiscal Year Final Research Report

• Project/Area Number: 25462880
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Aichi Gakuin University
• Principal Investigator: Nagano Keiji 愛知学院大学, 歯学部, 講師 (60367620)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 歯周病 / 歯肉上皮細胞 / 嫌気培養 / 付着 / トレポネーマ / 運動 / べん毛

Outline of Final Research Achievements

Periodontal disease is caused by multi-species of bacteria colonizing the periodontal tissues. Some species directly adhere to the gingival epithelial cells, but the mechanism is not well understood. Periodontitis-associated bacteria generally grow in an oxygen-free (anaerobic) environment. However, studies on adhesion to gingival epithelial cells have been performed under aerobic conditions. Therefore, in this study, it was conducted to establish a method of the infection experiment under anaerobic conditions. In addition, several strains of periodontitis-associated and anaerobic bacterium Treponema denticola were comparatively analyzed for some typical pathogenicities including adhesive activity to the gingival epithelia.

Free Research Field

微生物学