Expression and functional analysis of MSX homeobox gene family in dental pulp

2015 Fiscal Year Final Research Report

• Project/Area Number: 25462889
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional basic dentistry
• Research Institution: Hiroshima University
• Principal Investigator: FUJIMOTO KATSUMI 広島大学, 医歯薬保健学研究院(歯), 助教 (40294566)
• Research Collaborator: GOTO Noriko 広島大学, 病院(歯), 歯科診療医 (10735137)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 歯髄細胞 / MSX1 / 骨芽細胞分化 / 象牙芽細胞分化

Outline of Final Research Achievements

We examined that the role of MSX1 in regulating osteogenic/odontogenic differentiation of dental pulp stem cells (DPSCs). Knockdown of MSX1 suppressed the expression of osteoblast-related genes, alkaline phosphatase activity and calcification in DPSCs under osteogenic conditions, suggesting that MSX1 positively regulates osteogenic/odontogenic differentiation. MSX1 proteins were intensely expressed in odontoblasts of the roots and pulpal mesenchymal cells surrounding the blood vessels in the maxillary molar of 3 week-old mice. In addition, we performed gene expression microarray and ChIP-seq analysis to identify candidate MSX1 target genes.

Free Research Field

口腔生化学