Analysis of dipeptidyl peptidase gene group

2015 Fiscal Year Final Research Report

• Project/Area Number: 25462893
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional basic dentistry
• Research Institution: Nagasaki University
• Principal Investigator: KOBAYAKAWA Takeshi 長崎大学, 医歯薬学総合研究科(歯学系), 技術専門職員 (10153587)
• Co-Investigator(Kenkyū-buntansha): NEMOTO Takayuki K 長崎大学, 医歯薬学総合研究科(歯学系), 教授 (90164665) ; NEMOTO Yuko (OHARA Yuko) 長崎大学, 医歯薬学総合研究科(歯学系), 准教授 (10164667)
• Co-Investigator(Renkei-kenkyūsha): KIMURA Shigenobu 岩手医科大学, 歯学部, 教授 (10177917)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 歯周病原性細菌 / Porphyromonas gingivalis / レサズリン / 蛍光 / アミノ酸代謝 / トランスポーター

Outline of Final Research Achievements

We investigated the mechanism of amino acid-uptake in a periodontopathic bacterium, Porphyromonas gingivalis. Bacterial growth was markedly reduced in the 4 dpp genes disrupted strain. This result indicated that the DPP activity plays a crucial role in the bacterial growth and energy metabolism. We revealed that the bacterial metabolic activity and amino acid uptake could be measured by the analysis with Resazurin. By using this procedure, we found that P. gingivalis incorporated dipeptides as the nutrient, and that this route is indispensable for the growth of this bacterium.

Free Research Field

口腔生化学