2015 Fiscal Year Final Research Report
Functional analysis of the pioneer transcription factor FoxO1 in keratinocytes
| Project/Area Number |
25462906
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Fukuoka Dental College |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | FoxO1 / 遺伝子発現 |
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| Outline of Final Research Achievements |
In this study, we generated a tetracycline-inducible FoxO1 expressing keratinocyte cell line (Tet-On Ty1-FoxO1 cells), in order to clarify the functional roles of FoxO1 in keratinocytes. Tet-On Ty1-FoxO1 cells were treated with doxycycline to induce the expression of FoxO1, and then subjected to a global gene expression analysis. We identified FoxO1-regulated genes in keratinocytes, and confirmed the FoxO1 binding to genomic DNA of the target genes. Furthermore, we found that 3-deazaneplanocin A reduced the protein levels of FoxO1 in an oral squamous cell carcinoma cell line.
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| Free Research Field |
歯科薬理学
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