The effects of PKR and NLRP3 inflammasome on periodontal diseases

2015 Fiscal Year Final Research Report

• Project/Area Number: 25462918
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pathobiological dentistry/Dental radiology
• Research Institution: The University of Tokushima
• Principal Investigator: YOSHIDA Kaya 徳島大学, 大学院医歯薬学研究部, 講師 (60363157)
• Co-Investigator(Kenkyū-buntansha): OKAMURA Hirohiko 徳島大学, 大学院医歯薬学研究部, 准教授 (20380024)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: PKR / インフラマソーム / 歯周病 / 骨芽細胞

Outline of Final Research Achievements

To investigate the roles of PKR and NLRP3 inflammasome on the progress of periodontal diseases, we examine whether PKR affect NLRP3 and bone metabolisms in P. gingivalis-infected osteoblasts.
P. gingivalis increased the expression and phosphorylation of PKR in mouse osteoblasts, MC3T3-E1. P. gingivalis also induced and activated NLRP3, resulting in the increase of caspase-1 cleavage and interleukin (IL)-1b release. PKR was necessary to the up-regulation of NLRP3. Moreover, P. gingivalis affects the expression of several bone-related genes through by PKR-dependent pathway. These results showed that PKR mediates bone metabolisms by affecting NLRP3 and bone-related genes in periodontal diseases.

Free Research Field

口腔組織学