Glycoprotein of intact plasma membrane determines the diffusion properties of cellular water

2015 Fiscal Year Final Research Report

• Project/Area Number: 25462924
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pathobiological dentistry/Dental radiology
• Research Institution: Nagasaki University
• Principal Investigator: EIDA Sato 長崎大学, 医歯薬学総合研究科(歯学系), 助教 (80325662)
• Co-Investigator(Kenkyū-buntansha): HOTOKEZAKA Yuka 長崎大学, 病院(歯学系), 講師 (10244089) ; KATAYAMA Ikuo 長崎大学, 医歯薬学総合研究科(歯学系), 助教 (80295089) ; SASAKI Miho 長崎大学, 病院(歯学系), 助教 (10437874) ; NAKAMURA Takashi 長崎大学, 医歯薬学総合研究科(歯学系), 教授 (30172406)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 細胞膜 / 糖蛋白 / ADC

Outline of Final Research Achievements

Using an in vitro system and a high-resolution MR imaging technique, we show that the length of the intact plasma membrane is a major determinant of water diffusion in a controlled cellular environment and that the cell perimeter length (CPL) is sufficient to estimate the apparent diffusion coefficient (ADC) of water in any cellular environment in our experimental system (ADC=-0.21 × CPL + 1.10). We used this finding to further explain the different diffusion kinetics of cells that are dying via apoptotic or necrotic cell death pathways exhibiting characteristic changes in size, nuclear and cytoplasmic architectures, and membrane integrity. These results suggest that the ADC value can be used as a potential biomarker for cell death.

Free Research Field

医歯薬学