2015 Fiscal Year Final Research Report
Development of detection system of oncogene expression
| Project/Area Number |
25462934
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pathobiological dentistry/Dental radiology
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| Research Institution | Nihon University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 遺伝子変異 / 新規遺伝子増幅法 / EGFR / KRAS / がん / スクリーニング |
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| Outline of Final Research Achievements |
We developed detection systems for EGFR-1 and KRAS gene mutation. In addition, we attempted detection of these gene mutations from oral swab specimen.
We designed primer sets for each gene mutations, and investigate several cell lines. The result showed that the system detected gene mutations within 20 minutes. However, oral swab failed detection of these gene mutations. We simulate the number and preparation conditions in cell line, which resulted lower detection limit (10-100 cells). These data indicate that there are very few mutation positive cases or it is difficult to detect from keratinized cells.
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| Free Research Field |
病態科学系歯学・歯科放射線学
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