Monomer-collagen interactions studied by saturation transfer difference NMR.

2016 Fiscal Year Final Research Report

• Project/Area Number: 25462951
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: Hiraishi Noriko 東京医科歯科大学, 大学院医歯学総合研究科, 日本学術振興会特別研究員 (20567747)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: NMR / コラーゲン / 象牙質 / 接着性モノマー / 疎水性結合

Outline of Final Research Achievements

The nteraction of functional adhesive monomers with collagen is not understood at a molecular/atomic level. We performed saturation transfer difference (STD) NMR spectroscopy to investigate the binding interaction of 10-methacryloyloxydecyl dihydrogenphosphate (MDP), with atelocollagen as a triple-helical peptide model. High STD intensities were detected on the protons in the aliphatic region in MDP. The STD results imply that MDP has a relatively stable interaction with the collagen, because of the hydrophobic interactions between the hydrophobic MDP moieties and the hydrophobic collagen surface. This finding indicates that MDP-collagen complexation accounts for stable dentin bonding. Furthermore, we examined molecular-level interaction of MDP with dentin components by solid-state 31P NMR spectroscopy using a light-cured commercial available MDP-based adhesive. MDP-mineral reaction/binding was confirmed in cured adhesive with dentin. Interaction with collagens needs further studies.

Free Research Field

歯科保存修復学