Regulation of AChR cluster formation by miR-206 in masticatory muscles of mi/mi mice.

2015 Fiscal Year Final Research Report

• Project/Area Number: 25463037
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Prosthodontics/ Dental materials science and
• Research Institution: Tsurumi University
• Principal Investigator: SUGA Takeo 鶴見大学, 歯学部, 講師 (40247333)
• Co-Investigator(Kenkyū-buntansha): OGAWA Takumi 鶴見大学, 歯学部, 教授 (20267537) ; ANDO Hitoshi 鶴見大学, 歯学部, 講師 (00282765)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: miR-206 / miR-29 / mi/mi mouse / 咬筋

Outline of Final Research Achievements

Expression of endogenous miR-206 markedly increased with a peak at 4 weeks in the masseter of wild mice, but it kept low level until 12 weeks in those of the mi/mi mice. It suggested that miR-206 has a role in the formation of ACh receptor clusters. However, The average number of ACh receptor clusters was not changed when expression of miR-206 was inhibited or enhanced. More successful method of loss and gain of function experiments for microRNAs. We also investigated expression change of miR-29a which is another important microRNA in mygenesis. Expression pattern of miR-29a was similar to miR-206, but it could not change expressions of Agrin, MuSK, Lrp4 and Rapsin, which are regulation genes of ACh reseptor cluster formation.

Free Research Field

医歯薬学