Study of embryotoxicity using the iPS/ES cells of corrosion of dental silver alloy in human saliva

2015 Fiscal Year Final Research Report

• Project/Area Number: 25463040
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Prosthodontics/ Dental materials science and
• Research Institution: Osaka Dental University
• Principal Investigator: IMAI Koichi 大阪歯科大学, 歯学部, 教授 (90103100)
• Co-Investigator(Renkei-kenkyūsha): TAKEDA Shoji 大阪歯科大学, 歯学部, 名誉教授 (20067185)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 歯科用銀合金 / ES-D3細胞 / マウスiPS細胞 / 発生毒性 / 腐食 / 唾液 / 口腔 / 酸性環境

Outline of Final Research Achievements

The effects of the corrosion products of dental silver and gold- and silver-palladium alloys in the mouth on human development remain unclear. Metal ion compositions, such as silver, copper, and tin, are known to exhibit low-level embryotoxicity. The variations in embryotoxicity should be examined for the corrosion products of alloy surfaces. Using the Embryo Stem Cell Test (EST) protocol, the teratogenicity of unknown chemical substances in human fetuses can be predicted with mouse ES and 3T3 cells. In the present study, mouse iPS and ES cells were used. As a result, there was no significant difference in embryotoxicity between alloy surfaces treated or untreated with human or artificial saliva. However, mechanical scratching and subsequent immersion in hydrochloric and sulfuric acids markedly facilitated the elution of alloy elements from alloy surfaces, raising concerns about embryotoxicity.

Free Research Field

歯科理工学