In vitro investigation for future development to evaluate gingiva quality

2016 Fiscal Year Final Research Report

• Project/Area Number: 25463044
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Prosthodontics/ Dental materials science and
• Research Institution: Fukuoka Dental College
• Principal Investigator: Sato Hironobu 福岡歯科大学, 口腔歯学部, 教授 (00145955)
• Research Collaborator: Seo Akira 福岡歯科大学, 大学院生 ; Miyazono Shoji 福岡歯科大学, 大学院生
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: 歯肉 / 角化 / 上皮 / ケラチノサイト / 三次元培養 / カルシウム / KGF / アスコルビン酸

Outline of Final Research Achievements

Gingival recession is a frequent symptom and disturbs the patients' satisfaction, a cause of which is believed quality of gingiva. As thick and highly keratinized gingiva withstands recessing, 3D culture system of keratinocyte could be useful to detect the factors determining thickness and keratinization of gingival epithelium and to develop the future diagnostic method of gingival quality. We have established a 3D culture model using keratinocytes alone creating a proper keratinized epithelial equivalent, and furthere have identified some essential factors to produce the equivalent. Calcium is the essential for stratification and differential of keratinocytes. Addition of keratinocyte growth factor (KGF), but not ascorbic acid, prolongs a life span. Further addition of ascorbic acid improves the tissue architecture in cooperation with KGF.

Free Research Field

歯科補綴学