2015 Fiscal Year Final Research Report
The manufacture of the regeneration bone by the minimum differentiation induction of iPS cells and application to a pig defect of jaw bone model
| Project/Area Number |
25463071
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
HOSHI Kazuto 東京大学医学部附属病院, 口腔外科, 准教授 (30344451)
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| Co-Investigator(Renkei-kenkyūsha) |
TAKATO Tsuyoshi 東京大学医学部附属病院, 口腔外科, 教授 (90171454)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | iPS細胞 / 骨再生 |
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| Outline of Final Research Achievements |
The purpose of this study is to apply iPS cells to regenerative medicine for jawbone. We attempt to culture iPS cells from a miniature pig (piPSC) with minimum differentiation induction toward mesodermal cells and confirm the safety and reliability of the cells as a cell source by investigating the risk of tumorigenesis. In this study, we adopted clawn miniature pigs as inbred strains suitable for allogenic transplantation and established so called “iPS-like cells” by introducing the Yamanaka factors (Oct3/4, Sox2, Klf4, c-Myc) into miniature pig embryonic fibroblasts using virus vectors. Afterwards, the cells were cultured in serum-free medium containing Activin A and BMP-4 and differentiated into mesodermal cells. Finally, we examined purification methods of the differentiation-induced cells by removing undifferentiated cells.
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| Free Research Field |
再生医学
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