2015 Fiscal Year Final Research Report
Application to oral cancer treatment of nanobubbles and sonication
| Project/Area Number |
25463096
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Kyushu Dental College |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | ソノポレーション / ナノバブル / マイクロバブル / ドラッグデリバリー / 口腔癌 |
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| Outline of Final Research Achievements |
Ultrasound creates transient permeability of cell membranes in the presence of nanobubbles or microbubbles, and foreign molecules can easily enter cells, which is known as ‘sonoporation’. We developed anti-Epitherial Growth Factor Receptor (EGFR) antibody conjugated MBs (EGFR-MB). We examined the effect of EGFR-MB on oral squamous cell carcinoma. MBs were conjugated with an anti-EGFR antibody, and were entrapped ultrasound contrast gas. Ca9-22, a human gingival carcinoma cell line, were exposed to ultrasound with EGFR-MB and anti-cancer drug bleomycin (BLM). Sonoporation and EGFR-MB had a strong cytotoxic effect on Ca9-22 cells at the low dose of BLM. In addition, sonoporation and EGFR-MB remarkably increased the population of apoptotic cells even at a low dose BLM.Treatment with EGFR-MB and ultrasound make it possible to administer drugs into the cells more efficiently and specifically, suggesting a novel application for chemotherapy treatment for oral squamous cell carcinoma.
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| Free Research Field |
口腔外科
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