Shh signaling in pharyngeal development

2015 Fiscal Year Final Research Report

• Project/Area Number: 25463130
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: OKUHARA Shigeru 東京医科歯科大学, 医歯(薬)学総合研究科, 非常勤講師 (10451973)
• Co-Investigator(Kenkyū-buntansha): SATO Yutaka 東京医科歯科大学, 大学院医歯学総合研究科, 助教 (90361716) ; ISEKI Sachiko 東京医科歯科大学, 大学院医歯学総合研究科, 教授 (80251544) ; HARADA Kiyoshi 東京医科歯科大学, 大学院医歯学総合研究科, 教授 (30228639)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 口蓋裂 / 舌

Outline of Final Research Achievements

Compound deletion heterozygote for Sonic hedgehog gene and its enhancer MFCS4 was utilized as a cleft palate model mouse. In this mouse, development of lingual tendon was impaired, suggesting the lingual motion was insufficient to associate with palate development. We also found that neural crest derived craniofacial organs such as soft palate, epiglottis, arytenoid, thyroid cartilage, part of the bones and cartilages in cranial base, and extympanic bone are hypoplastic, suggesting that Shh signaling is required for neural crest derived mesenchymal cell condensation. Our findings also propose that the variant in enhancer sequence might be related to cleft palate.

Free Research Field

発生生物学