Signal-transmission mechanism for micro-damaged bone regeneration

2015 Fiscal Year Final Research Report

• Project/Area Number: 25463144
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Health Sciences University of Hokkaido
• Principal Investigator: Murata Masaru 北海道医療大学, 歯学部, 准教授 (00260662)
• Co-Investigator(Kenkyū-buntansha): ITO Katsutoshi 北海道医療大学, 歯学部, 助教 (50433438) ; AKAZAWA Toshiyuki 地方独立行政法人北海道立総合研究機構, 工業試験場, 研究主幹 (80469692)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 骨微小損傷 / 骨再生 / 亀裂 / 電解水 / 表面積

Outline of Final Research Achievements

Mouse temporal bone was harvested, and cut into fragments (5x5x1mm). The bone fragment was demineralized as EW-bone group by ultrasonic irradiation at 120W and 38 kHz for 20 min in the acidic EW (pH 2.7, 1.0 liter). The EW-bone was implanted into subcutaneous tissues of nude-mouse and explanted at 6 weeks. The specimens before and after implantation were observed by scanning electron microscope (SEM) and optical microscope. The EW-bone showed clear enlargement and union of cracks by SEM. The EW-bone revealed active bone induction over wide areas at 6 weeks. The original dead bone was covered with newly formed bone, and many active osteoblasts differentiated in the original marrow spaces.
The EW-bone had better performance in bone induction than the DW-bone. Our micro-damage technique with the combination of the acidic EW (pH2.7) and the ultrasonic irradiation will contribute to the modification (surface area, 3D structure) of the dense bone and the active bone induction.

Free Research Field

骨再生学