2015 Fiscal Year Final Research Report
Basic research for establishment of new therapy for acceralation of bone augumentation
| Project/Area Number |
25463157
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Tsurumi University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
FUJIHARA Hisako 鶴見大学短期大学部, 歯科衛生科, 准教授 (80396746)
YAMADA Hiroyuki 岩手医科大学, 歯学部, 准教授 (90267542)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 間葉系幹細胞 / 骨芽細胞 / ポリADP-リボシル化 / 分化 / 骨造成 |
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| Outline of Final Research Achievements |
Poly(ADP-ribosyl)ation is known to be involved in a variety of cellular processes by poly(ADP-ribose) polymerase (PARP). PARP involvement in MSCs (Mesenchymal Stem Cells) differentiation has been reported suggesting possible side effects of PARP inhibitors on MSCs. Therefore, effects of PARP inhibitors on MSCs were examined in this study. MSCs showed suppressed osteogenic differentiation after 1 μM PJ34 treatment without showing cytotoxicity, while differentiation of MSCs into chondrocyte or adipocyte was not affected. The mRNA induction of osteogenic markers such as and regulators of transcription factors was suppressed by PJ34 treatment. Extracellular mineralized matrix formation was also suppressed. These results strongly suggest that PARP inhibitors would suppress osteogenic differentiation and poly(ADP-ribosyl)ation may play a physiological role in osteogenic differentiation by regulating BMP-2 signaling pathway.
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| Free Research Field |
口腔外科
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