Induction of neural stem cells and possible contribution to neurogenesis following transient brain ischemia

2016 Fiscal Year Final Research Report

• Project/Area Number: 25463159
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Osaka Dental University
• Principal Investigator: MOMOTA Yoshihiro 大阪歯科大学, 歯学部, 教授 (60247880)
• Co-Investigator(Renkei-kenkyūsha): MAEDA Mitsuyo 理化学研究所, CLST-JEOL連携センターマルチモダル微細構造解析ユニット, 客員主管研究員 (40122080) ; MATSUYAMA Tomohiro 兵庫医科大学, 医学部, 教授 (10219529)
• Research Collaborator: NAKATA Masayo
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: 神経幹細胞 / ペリサイト / 一過性脳虚血 / 再灌流傷害

Outline of Final Research Achievements

Using a mouse model of transient ischemia/reperfusion injury, we investigated the threshold of ischemic periods. In addition, we examined the origin of injury-induced neural stem cells: iNSCs and their possible contribution to neurogenesis under such pathologic conditions. Although iNSCs were induced following not only lethal ischemic injury by more than 30-min ischemia but also non-lethal ischemic injury by 15-min ischemia, the numbers of iNSCs were fewer under 15-min ischemic conditions compared with those observed under 30-min ischemic conditions. We also found that iNSCs are likely derived from brain pericytes stimulated by ischemic insult and that they had an activity of producing neuronal cells.

Free Research Field

歯科麻酔学