Lysophosphatidic acid is involved in a cellular signaling of human periodontal ligament and tooth resorption

2016 Fiscal Year Final Research Report

• Project/Area Number: 25463196
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Orthodontics/Pediatric dentistry
• Research Institution: Health Sciences University of Hokkaido
• Principal Investigator: okayama miki 北海道医療大学, 歯学部, 助教 (30382500)
• Co-Investigator(Renkei-kenkyūsha): Ikeda kazutaka 国立研究開発法人理化学研究所, 統合生命医科学研究センター, 上級研究員 (10466732)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: リゾリン脂質 / ヒト歯根膜 / 歯根吸収

Outline of Final Research Achievements

We analyzed the expression of LPA receptors, synthetic enzymes of LPA and the intracellular signaling by LPA in human periodontal ligament fibroblasts (hPDLFs). The results showed a strong expression of LPA1 , LPA6 , and lyso－
PLD in hPDLFs. There was LPA induced phosphorylation of ERK1/2 in the hPDLFs. We also analyzed the expression of LPA receptors in mouse tooth germs at the cap and bell stages by RT－PCR, and found that all LPA receptors were expressed during tooth development.Further, we also analyzed LPA1 in mouse tooth germs using in situ hybridization and determined LPA1 expression in the dental mesenchyme at the initial stage and in both the mesenchyme and epithelium from the bud to the bell stages. These results suggest that LPA signaling would play an important role in tooth development and periodontal ligament functions.

Free Research Field

歯科矯正学