2015 Fiscal Year Final Research Report
BDNF regulates inflammatory cytokine expressions through TrkB in human vascular endothelial cells
| Project/Area Number |
25463218
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Periodontology
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| Research Institution | Hiroshima University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
Shiba Hideki 広島大学, 医歯薬保健学研究院(歯), 教授 (60260668)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 脳由来神経栄養因子 |
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| Outline of Final Research Achievements |
Brain-derived neurotrophic factor (BDNF) enhances periodontal tissue regeneration. Tissue regeneration is characterized by inflammation which directs the quality of tissue repair. In this study, we investigated the effect of BDNF on intercellular adhesion molecule (ICAM)-1, which is an inflammatory marker, expression in interleukin (IL)-1β-treated human microvascular endothelial cells (HMVECs). We demonstrate that BDNF attenuates the IL-1β-increased ICAM-1 mRNA and protein expressions. In addition, we studied the effect of BDNF on neutrophil-like differentiated HL-60 cells adhesion to HMVECs by cell adhesion assay. Treatment of HMVECs with IL-1β led to an increase in the number of adherent neutrophil-like HL-60 cells. BDNF decreased significantly the number of neutrophil-like HL-60 cells attached HMVECs. In conclusion, BDNF may reduce excess inflammation through reduced neutrophil recruitment by regulating the ICAM-1 expression.
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| Free Research Field |
歯周病学
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