Reconstruction of oral microflora by riboswitch regulation

2015 Fiscal Year Final Research Report

• Project/Area Number: 25463251
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Social dentistry
• Research Institution: Kyushu University
• Principal Investigator: Shibata Yukie 九州大学, 歯学研究科(研究院), 助教 (30274476)
• Co-Investigator(Kenkyū-buntansha): Yamashita Yoshihisa 九州大学, 大学院歯学研究院, 教授 (20192403)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: フッ化物 / 口腔細菌 / eriC / crcB

Outline of Final Research Achievements

It has been reported that eriC and crcB were involved in the bacterial fluoride resistance. Blast research demonstrated that two kinds of eriCs (eriC1 and eriC2) and two kinds of crcBs (crcB1 and crcB2) existed in 18 oral streptococci. They were divided into three groups on the basis of the distribution of these four genes: group I, only eriC1; group II, eriC1 & eriC2; group III, eriC2, crcB1, & crcB2. Group I consisted of Streptococcus mutans, and one of two eriC1s dominantly affected the fluoride resistance. Group II consisted of 8 species, and eriC1 was involved in fluoride resistance, but eriC2 was not, in Streptococcus anginosus as a representative species. Group III consisted of 9 species, and both crcB1 and crcB2 were indispensable for the fluoride resistance, but eriC2 was not, in Streptococcus sanguinis as a representative species. Base on these results, either EriC1 or CrcB works for the fluoride resistance in oral streptococci.

Free Research Field

予防歯科学