2016 Fiscal Year Final Research Report
Multiomics analysis to reveal the functional meaning of DNA methylation in Neurospora crassa
| Project/Area Number |
25503001
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
epigenetics
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Keywords | Neurospora crassa / DNA methylation / Histone modification |
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| Outline of Final Research Achievements |
To reveal the functional meanings of DNA methylation in Neurospora crassa, a series of comparative analyses was performed among wild type and three mutants with deficient or showing altered DNA methylation. By using whole-genome bisulfite sequencing (WGBS) and chromatin immunoprecipitation combined with massive parallel sequencing (ChIP-Seq), genome-wide states for DNA methylation and histone post-translational modification were compared. We observed some interesting relationships between histone modifications and DNA methylation, which indicates the existence of novel function of DNA methylation on histone modifications.
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| Free Research Field |
Epigenetics
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