Role of RNA molecules in the polycomb complex, PRC1

2015 Fiscal Year Final Research Report

• Project/Area Number: 25503004
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: epigenetics
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Masui Osamu 国立研究開発法人理化学研究所, 統合生命医科学研究センター, 研究員 (30579305)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: ポリコーム複合体 / RNA / Xist

Outline of Final Research Achievements

In this study, we tried to address how Polycomb repressive complex 1 (PRC1) suppresses its target genes via RNA molecule(s). We performed the RNA immuno-precipitation (RIP) assay using the antibody against Ring1B protein that is a catalytic subunit of PRC1, and discovered (1) PRC1 (Ring1B) binds to Xist RNA and mRNAs that code components of PRC1 itelf, and (2) the binding between PRC1 and these RNAs is not mediated by Ring1B or Cbx proteins but an unknown 120 kDa protein.

Free Research Field

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