2015 Fiscal Year Final Research Report
In vivo analysis of the canalicular trafficking of lipid transporters in lithogenic diet-fed mouse.
| Project/Area Number |
25504008
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Integrated Nutrition Science
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| Research Institution | University of Shizuoka |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 肝毛細胆管 / 細胞内トラフィキング / ＡＢＣトランスポーター / 高脂肪食 / cAMPシグナル |
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| Outline of Final Research Achievements |
ABC half transporters Abcg5 and Abcg8, which form heterodimer in the liver bile canaliculi, promote the secretion of cholesterol into bile. Hyperfunction of these transporters causes the formation of cholesterol gallstones in the bile. We previously demonstrated diet-induced hypernutrition caused a significant increase in the function and expression of these transporters in bile canaliculi, the mechanisms underlying this phenomenon remained to be clarified. In this study, we investigated how hypernutrition affects the canalicular trafficking and the function of Abcg5/Abcg8. As a result, we clarified that diet-induced cholesterol loading into liver accelerates the trafficking of Abcg5/Abcg8 from the intrahepatic pools to the bile canalicular membrane through cAMP signaling machinery.
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| Free Research Field |
医歯薬学
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