2015 Fiscal Year Final Research Report
Proposed model that integrates cholesterol bidirectional flux in the small intestine
| Project/Area Number |
25504013
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Integrated Nutrition Science
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| Research Institution | Saitama Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
TAKENAKA Yasuhiro 埼玉医科大学, 医学部, 研究員 (20586789)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | コレステロール / 小腸 / 吸収 / コレステロール逆輸送 |
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| Outline of Final Research Achievements |
Ezetimibe inhibits Niemann-Pick C1-like 1 (NPC1L1), thereby reduces intestinal cholesterol absorption. This treatment also increases extrahepatic reverse cholesterol transport via an undefined mechanism. To explore this, we employed a trans-intestinal cholesterol efflux (TICE) assay and found an increase of TICE by 45%. To examine whether such increase in efflux occurs at the intestinal brush border membrane(BBM)-level, we performed luminal perfusion assays, similar to TICE but the jejunal wall was labelled with orally-given 3H-cholesterol, and determined elevated BBM-to-lumen cholesterol efflux by 3.5-fold with ezetimibe. Such increased efflux probably promotes circulation-to-lumen cholesterol transit eventually; thus increases TICE.
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| Free Research Field |
病態生化学
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