Energy-resolved tandem mass spectrometry and ion mobility spectrometry for identification and isomer differentiation

2016 Fiscal Year Final Research Report

• Project/Area Number: 25513011
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: オミクス計測科学
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Nakamura Takemichi 国立研究開発法人理化学研究所, 環境資源科学研究センター, 専任研究員 (10360611)
• Co-Investigator(Renkei-kenkyūsha): TAKAHASHI Shunya 国立研究開発法人理化学研究所, 環境資源科学研究センター, 専任研究員 (00202151)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: 異性体識別 / フラグメンテーション / 衝突活性化 / エネルギー分解法 / タンデム質量分析法 / オミクス計測科学 / ジアステレオマー

Outline of Final Research Achievements

We have shown that breakdown diagrams obtained by energy-resolved tandem mass spectrometry (ER-MS/MS) experiments are useful for identification of small organic molecules and differentiation of isomers including diastereomers, such as Thr and allo-Thr, as well. The established ER-MS/MS method is based on intentional observation of energy dependence in fragmentation chemistries and fast and sensitive enough to be used in the context of omics molecular identification. Our strategy would leads to an innovation in molecular identification in future omics sciences where isomer differentiation will become an even bigger challenge under mass imaging, single cell analysis, and/or ambient in situ ionization setups where no chromatography is available.

Free Research Field

質量分析学