Detection of peptides in atmospheric organic aerosols of marine microbial origin

2014 Fiscal Year Final Research Report

• Project/Area Number: 25550003
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Environmental dynamic analysis
• Research Institution: Hokkaido University
• Principal Investigator: MIYAZAKI Yuzo 北海道大学, 低温科学研究所, 助教 (60376655)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: アミノ酸 / 大気エアロゾル / 海洋微生物 / ペプチド / アミノ酸含有有機物 / 誘導体化 / タンパク質

Outline of Final Research Achievements

An analytical method was established for the determination of both free and combined amino acids including peptide in marine atmospheric aerosols. The method derivatized amino acids followed by a gas chromatography-mass spectrometry (GC-MS) analysis. The application of the adopted method in marine aerosols of the western North Pacific and the equatorial Pacific enabled the identification and quantification of 16 amino acids. Glycine was the predominant amino acids for both free and combined form in the aerosols originated from sea surface. On average, the total concentration of combined amino acids was found to be four to five times higher than that of free amino acids in the aerosol samples.

Free Research Field

大気化学