Generation of ES cells to meseare DNA repair activity with reporter genes

2014 Fiscal Year Final Research Report

• Project/Area Number: 25550026
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Risk sciences of radiation and chemicals
• Research Institution: Kyoto University
• Principal Investigator: KOBAYASHI Junya 京都大学, 放射線生物研究センター, 准教授 (30301302)
• Co-Investigator(Kenkyū-buntansha): HONDA Hiroaki 広島大学, 原爆放射線医科学研究所, 教授 (40245064) ; KATO Akihiro 京都大学, 放射線生物研究センター, 研究員 (70423051) ; KOMATSU Kenshi 京都大学, 放射線生物研究センター, 教授 (80124577)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: DNA修復 / 相同組換え / 非相同末端結合 / 放射線 / DNA損傷

Outline of Final Research Achievements

In order to investigate radiosensitivity in children, we generated the system in ES cells to measure the activity of homologous recombination (HR) repair or non-homologous endjoining (NHEJ) repair. We introduce a specific site of genomic DNA to HR or NHEJ reporter construct. As a result, we got several ES cell lines, which could express NHEJ after generation of DNA double-strand break and possess differentiation potency.

Free Research Field

放射線生物学