2014 Fiscal Year Final Research Report
Development of an endotoxin detection system using IL-8 reporter cell
| Project/Area Number |
25550076
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Environmental risk control and evaluation
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | エンドトキシン / ルシフェラーゼアッセイ / 環境水 / 免疫撹乱 |
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| Outline of Final Research Achievements |
So far, the assay system which can detect contamination of microorganism-derived toxin such as endotoxin to environmental water comprehensively, sensitively and rapidly have not been developed. Our aim was to develop assay system widely screening microorganism-derived toxin, using a THP-1 monocyte cell line derived IL-8 reporter cell, THP-G8.
The environmental water around Sendai, Japan and Manila, Phillipine induced IL-8 reporter activity, however, these induction were not completely correlated with the result measured by LAL method, suggesting that immunodisturbance except endotoxin related to the induction and that THP-G8 could access these activity. Using the suppression index by TLR4-antagonist and anti-oxidant, the environmental water could be divided to several groups and could be estimated qualitatively and quantitatively.
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| Free Research Field |
免疫学
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