Improvement of nutritional properties in soybean by branched amino acid signaling

2014 Fiscal Year Final Research Report

• Project/Area Number: 25560038
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Eating habits
• Research Institution: University of Miyazaki
• Principal Investigator: YUASA Takashi 宮崎大学, 農学部, 教授 (40312269)
• Co-Investigator(Kenkyū-buntansha): INOUE Mari (IWAYA Mari) 九州大学, 農学研究科点, 教授 (60001394)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: アミノ酸 / 栄養シグナル / オートファジー / ダイズ / 転写因子 / ATG8 / BCAT / bZIP型転写因子

Outline of Final Research Achievements

Sucrose starvation treatment signi,cantly enhanced the expressions of GmbZIP53A, but not GmbZIP53B asparagine synthase (GmASN), proline dehydrogenase (GmProDH), and branched chain amino acid transaminase (GmBCAT). GmbZIP53-related immunoreactive signals were upregulated under severe starvation with sucrose starvation and protease inhibitors, while sucrose and sucrose starvation had no or marginal eects on the signal. Pro,les of induction of GmASN, GmProDH and GmBCAT3 under various nutrient conditions were consistent with the profles of GmbZIP53 protein levels but not with those of GmbZIP mRNA levels. These results indicate that GmbZIP53 proteins levels are regulated by posttranslational mechanism in response to severe starvation stress and that the increased protein of GmbZIP53 under severe starvation accelerates transcriptional induction of GmASN, GmProDH, and GmBCAT.

Free Research Field

作物分子生物学