2014 Fiscal Year Final Research Report
Fetal transfer mechanism of methyl donors across the placenta and its epigeneomes
| Project/Area Number |
25560063
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Eating habits
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| Research Institution | Keio University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
NISHIMURA Tomohiro 慶應義塾大学, 薬学部, 専任講師 (40453518)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | 胎盤関門 / トランスポーター |
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| Outline of Final Research Achievements |
We aimed to examine the fetal transfer mechanism of methyl donors, such as methionine, 5-methyltatrahydrofolate (MTHF), and betaine, across the placenta. LAT1, a transporter for methionine, was observed to be specifically localized at the brush-border membrane of rat placental syncytiotrophoblast layer I, suggesting the role of LAT1 in the fetal transfer of amino acids. Fetal transfer rate of 5-methyltatrahydrofolate (MTHF) and betaine appears to be low compared with methionine transported by LAT1, implying the importance of methionine in terms of placental permeability. SNAT2 mediates the transfer of betaine, and its functional expression in placental brush-border membrane was found to be detected in rat but not in human. This suggests the presence of the species difference in betaine transport. Methylated CpG level in the promoter region of the Abcg2 gene was observed to be very low.
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| Free Research Field |
食生活学
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