Tissue engineering on microvessel construction stabilized by pericyte covering

2014 Fiscal Year Final Research Report

• Project/Area Number: 25560208
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Keio University
• Principal Investigator: SUDO RYO 慶應義塾大学, 理工学部, 准教授 (20407141)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 血管新生 / 組織工学 / 三次元培養 / ペリサイト / マイクロ流体デバイス

Outline of Final Research Achievements

In this study, endothelial cells and mesenchymal stem cells were cocultured in a microfluidic device to construct stable microvasculature covered by pericytes. In particular, we investigated the role of pericytes in the process of vascular formation, and the three-dimensional structure of constructed microvascular networks covered by pericytes. We found culture conditions to promote capillary formation by quantitatively analyzing the interaction between endothelial cells and mesenchymal stem cells, and established a tissue engineering method for construction of stable microvascular networks covered by pericytes. These findings have an important implication for the development of tissue engineering to construct three-dimensional tissues including microvascular networks.

Free Research Field

生体組織工学