Physiomic analysis of the regulatory mechanism of cardiomyocyte

2014 Fiscal Year Final Research Report

• Project/Area Number: 25560214
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Kawasaki Medical School
• Principal Investigator: MOHRI Satoshi 川崎医科大学, 医学部, 教授 (00294413)
• Co-Investigator(Kenkyū-buntansha): HASHIMOTO Ken 川崎医科大学, 医学部, 講師 (80341080) ; UJIHARA Yoshihiro 川崎医科大学, 医学部, 助教 (80610021)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 心筋細胞 / 細胞分裂 / 酸素環境 / 細胞周期

Outline of Final Research Achievements

Cardiomyocytes are terminally differentiated cells that can not proliferate for repairing damaged tissues as with nerve cells. Regeneration medicine is a possible approach for these untreatable diseases, which is developing the techniques to create functional issues to repair the damaged organs. In this study, we focused on the changes in oxygen concentration during maternal period. In utero, partial oxygen pressure is about 20 mmHg and it increases to about 90 mmHg after birth because of the breathing activity. We tested the hypothesis that a dramatic increase in oxygen tension arrests the cell cycle of cardiocmyocytes. First, we visualized the dividing cultured cardiomyocytes using medial engineering technology and confirmed that cardiomyocytes arrested cell cycle when exposed to high oxygen tension. Then, we investigated the alteration of gene expression in this change of oxygen tension and performed silencing these genes to confirm the involvement to regulate cell cycle.

Free Research Field

循環生理学