Improvement of optical tweezers and development of micro cell culture wells for realization of sono-injection technique

2014 Fiscal Year Final Research Report

• Project/Area Number: 25560233
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Medical systems
• Research Institution: Hokkaido University
• Principal Investigator: KUDO Nobuki 北海道大学, 情報科学研究科, 准教授 (30271638)
• Co-Investigator(Kenkyū-buntansha): SHIGETOMI Kaori 北海道大学, 大学院情報科学研究科, 特任助教 (90431816)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: マイクロインジェクション / 超音波 / 微小気泡 / 光ピンセット / マイクロ流路

Outline of Final Research Achievements

The studies investigated the two elemental technologies that are essential for experimental studies on gene and drug transduction by exposure of cells with attached microbubbles to ultrasound. Since the transduction occurs at the position of the attached bubbles, its efficiency can be controlled by the size and position of the attached bubbles. Therefore, in this study, conventional optical tweezers were improved to increase bubble trapping force to move a bubble on a cell nucleus. Small cell culture wells of around 100 microns were also fabricated to maintain cultured cells under in vivo-like conditions that have high cell membrane repairability. Transduction experiments were carried out using the fabricated wells, indicating that the wells are useful for basic studies on gene and drug delivery by ultrasound exposure.

Free Research Field

医用システム