2014 Fiscal Year Final Research Report
Identification and genome analysis of bacterial symbiont producing sponge-derived secondary metabolites
| Project/Area Number |
25560399
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Biomolecular chemistry
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2013-04-01 – 2015-03-31
|
| Keywords | 海綿共生微生物 / 生物活性物質 / Discodermia / Theonella / 生合成 |
|---|
| Outline of Final Research Achievements |
In this study, we tried to identify the bacterial symbiont producing sponge-derived secondary metabolites. Theonellidae family sponges are well known to contain diverse secondary metabolites. However, the real producer of them had been unknown, though it had been supposed to be symbiont bacteria. The identification of such bacteria could be a key issue to pave the way for the supply limitation of medicinally important sponge-derived compounds. Three Theonellidae sponge species were therefore subjected to genetic evaluation, cell separation and single cell analysis. As a result, the Entotheonella was identified to be the real producer of almost all secondary metabolites of three sponge spieces. Our findings provide not only the proof of a sponge-specific molecule synthesized by the unique and uncultured bacterium rather than sponge itself, but also useful insights into the supply of sponge-derived medicinally important compounds.
|
| Free Research Field |
天然物化学
|
