Development of biosynthetic technology tools by using microbial secondary metabolism

2015 Fiscal Year Final Research Report

• Project/Area Number: 25560407
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biomolecular chemistry
• Research Institution: Tokyo University of Science
• Principal Investigator: HAYAKAWA Yoichi 東京理科大学, 薬学部, 教授 (20208606)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 生合成 / thioviridamide / チオアミド / prodigiosin / 側鎖環化遺伝子

Outline of Final Research Achievements

The genome analysis of a thioviridamide (TVA)-producing organism showed a gene encoding the TVA precursor peptide (tvaA). Heterologous production of TVA in Streptomyces lividans identified the thioviridamide biosynthetic gene cluster containing 12 genes (tvaA - tvaL). Prethioviridamide was extracted from the TVA producer with methanol as a true biosynthetic product, which was converted to TVA by acetone treatment.
In the rph gene cluster of the roseophilin producer, four genes including rphG showed homology to the prodigiosin cyclization gene redG. A redG disruptant of S. coelicolor expressing rphG produced two cyclized prodigiosins, metacycloprodigiosin and a new analogue, propyl-meta-cyclooctylprodiginine, suggesting that the rphG is a prodigiosin cyclization gene with novel regioselectivity.

Free Research Field

微生物二次代謝産物のケミカルバイオロジー