Studies on Ciona notochord tubulogenesis by chemical biology approach

2014 Fiscal Year Final Research Report

• Project/Area Number: 25560419
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Chemical biology
• Research Institution: Keio University
• Principal Investigator: IMOTO Masaya 慶應義塾大学, 理工学部, 教授 (60213253)
• Co-Investigator(Renkei-kenkyūsha): TASHIRO Etsu 慶應義塾大学, 理工学部, 専任講師 (00365446) ; HOTTA Kouji 慶應義塾大学, 理工学部, 専任講師 (80407147) ; OKA Koutaro 慶應義塾大学, 理工学部, 教授 (10276412)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: ホヤ / 脊索管形成

Outline of Final Research Achievements

In the present study, we aimed to elucidate the molecular mechanisms of Ciona notochord tubulogenesis by identifying the target protein of UTKO1, a cell migration inhibitor. Avidin beads pull down assay using biotin labeled UTKO1 and the lysate prepared by the larvae during notochord tubulogenesis identified the target protein of UTKO1, and we confirmed this direct interaction using the protein purified from E.Coli. Furthermore, knockdown of target protein using morpholino antisense oligonucleotide inhibited notochord tubulogenesis as seen in UTKO1 treatment, suggests the involvement of target in notochord tubulogenesis.

Free Research Field

ケミカルバイオロジー