2014 Fiscal Year Final Research Report
Quantitative localization of membrane molecules at nano-scale spatial resolution
| Project/Area Number |
25560425
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Basic / Social brain science
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| Research Institution | University of Fukui (2014)
Nagoya University (2013) |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | 電子顕微鏡 / 生体分子 / 分子局在 / 分子定量 |
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| Outline of Final Research Achievements |
Each of the biological phenomenon or functions of our body is generated by the interaction among molecules at subcellular and cellular levels. Thus, in order to deepen our knowledge about underlying mechanisms for the biological phenomenon and to develop new therapeutic approaches for diseases, it is important to uncover quantity (expression density) of individual bio-molecules at nano-scale spatial resolution. However, it is not possible to obtain such information by conventional molecular localization techniques. Here in this research project, I introduced several optimizations in SDS-digested freeze-fracture replica labeling and revealed localization of several ion channels in neuronal plasma membrane.
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| Free Research Field |
分子神経解剖学
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