Development of molecular manipulation in biological cells with surface plasmon resonance based optical trapping

2016 Fiscal Year Final Research Report

• Project/Area Number: 25600052
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Nanobioscience
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: Hosokawa Chie 国立研究開発法人産業技術総合研究所, バイオメディカル研究部門, 主任研究員 (60435766)
• Co-Investigator(Renkei-kenkyūsha): TAWA Keiko 関西学院大学, 理工学部, 教授 (80344109)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: 光ピンセット / ナノバイオ / 表面プラズモン共鳴 / 神経細胞

Outline of Final Research Achievements

We demonstrate surface plasmon resonance based optical trapping using a plasmonic chip toward realizing effective manipulation of molecules on the surface of neurons. Surface-plasmon-enhanced optical trapping was evaluated by the fluorescence analysis of nanoparticles suspended in water and neural cell adhesion molecules (NCAMs) labeled with quantum dots (Q-dots) on rat hippocampal neurons. The motion of nanoparticles in water and the molecular dynamics of NCAMs on neuronal cells cultured on a plasmonic chip were constrained at the laser focus more effectively than those on a glass substrate because of the surface plasmon resonance effect.

Free Research Field

応用物理学