A study on the on-chip transfection and time-lapse observation of initialization and differentiation

2014 Fiscal Year Final Research Report

• Project/Area Number: 25600056
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Nano/Microsystems
• Research Institution: The University of Tokyo
• Principal Investigator: WASHIZU Masao 東京大学, 工学(系)研究科(研究院), 教授 (10201162)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: マイクロ・ナノテクノロジー / エレクトロポレーション / マイクロチップ / 再生医療 / 静電気 / 遺伝子導入 / 細胞周期

Outline of Final Research Achievements

Gene transfection method on a microfabricated chip is established. The feature of the device, being able to precisely control the timing of transfection, is made use of to investigate the relation between cell cycle and initialization by Yamanaka factors. When the factors are fed to Hela cells recombined with Fucci factor (green fluorescence during G1 period, and red fluorescence through S-G2-M period), it was found that significantly large number of cells stopped at S-M period compared with that where no Yamanaka factors were fed. Staining with anti-Oct3/4 antibody, 70% of the cells showd simultaneous expression of GFP and Oct3/4. These observation suggest that the cell period stopping is induced by the feeding of, or the expression of Yamanaka Factors.

Free Research Field

バイオナノテクノロジー