High-throughput measurement of drug permeability through artificial lipid bilayers between nanoliter aqueous droplets

2015 Fiscal Year Final Research Report

• Project/Area Number: 25600058
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Nano/Microsystems
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: Nisisako Takasi 東京工業大学, 精密工学研究所, 助教 (10431983)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: ナノバイオ / マイクロ・ナノデバイス / 流体工学

Outline of Final Research Achievements

We studied formation of artificial planar lipid bilayers and their application in drug permeability assay by using microfluidic channels fabricated on a planar substrate. Nanoliter-sized aqueous donor and acceptor droplets were continuously formed at T- or cross-shaped channels into a lipid-containing organic stream. At the downstream, selective aspiration of the organic phase brought the aqueous donor and acceptor droplets into contact, thereby creating droplet interface bilayers (DIBs). Furthermore, we could rapidly measure passive membrane permeability of drug molecules across the formed DIBs.

Free Research Field

マイクロ・ナノデバイス