Preparation of Bioactive Janus Particles using Hydrogel Templates

2015 Fiscal Year Final Research Report

• Project/Area Number: 25600062
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Nano/Microsystems
• Research Institution: Saga University
• Principal Investigator: Narita Takayuki 佐賀大学, 工学(系)研究科(研究院), 准教授 (30423560)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 走化性 / JANUS状粒子

Outline of Final Research Achievements

In this study, we attempted to prepare bioactive Janus particles with two different bio-active parts via two methods using combination of alginate gel and gelatin gel, which immobile two proteins located in each gels separately. From the two-tone figure of fluorescence micrograph, Janus particles successfully prepared by the one for one emulsion collision methods, because the part of alginate gel particle was only FITC-labeled and yield fluorescence. In the enzyme assay for the immobilized glucose oxidase and catalase, we confirmed in both of particle systems that pH decrease was observed in the glucose solution and oxygen generate in the hydrogen peroxide. The results demonstrate that physical immobilization using polysaccharide and gelatine gel is reasonable to maintain high enzyme activities.

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