2014 Fiscal Year Final Research Report
Development of scanning ion-conductance microscopy for quantifying cell membrane fluctuation
| Project/Area Number |
25600127
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General applied physics
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
SUEOKA Kazuhisa 北海道大学, 情報科学研究科 (60250479)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | イオンコンダクタンス顕微鏡 / 細胞膜 / 揺らぎ |
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| Outline of Final Research Achievements |
Scanning ion-conductance microscopy (SICM) is a powerful tool for imaging topography of cell surfaces without their deformation at a high spatial resolution. The aim of this study is to develop SICM that enables us to quantify membrane fluctuations of adherent cells. Using micro-patterned substrates that enable to control cytoskeletal structures of cells, we investigated how the fluctuation of apical cell membrane is associated with the cytoskeletal structures. The micro-patterned substrate was fabricated on transparent glass substrate, so that the substrate allows us to obtain fluorescent image of intracellular structures. The results show no clear relationship between the cell membrane fluctuation and the actin filamentous structures of micro-patterned cells. Moreover, we investigated the scan speed dependence of ion-distance curves.
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| Free Research Field |
細胞生物物理
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