Quantification of methanogenic potential with coenzyme F430 analysis

2015 Fiscal Year Final Research Report

• Project/Area Number: 25610166
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Geochemistry/Cosmochemistry
• Research Institution: National Institute of Advanced Industrial Science and Technology (2015); Japan Agency for Marine-Earth Science and Technology (2013-2014)
• Principal Investigator: Kaneko Masanori 国立研究開発法人産業技術総合研究所, 地圏資源環境研究部門, 主任研究員 (80633239)
• Research Collaborator: OHKOUCH Naohiko 海洋研究開発機構, 生物地球化学分野, 分野長 ; TAKANO Yoshinori 海洋研究開発機構, 生物地球化学分野, 主任研究員 ; NARAOKA Hiroshi 九州大学, 理学研究院, 教授 ; SHIMA Seigo Max Planck Institutes, Microbial Protein Structure, Research Group Leader ; WATANABE Kenji 名古屋大学, 農学部, 講師
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 補酵素F430 / 機能特異分子分析 / メタン生成 / 嫌気的メタン酸化 / 液体クロマトグラフィートリプル四重極質量分析

Outline of Final Research Achievements

During the research period, analytical method was developed to quantify coenzyme F430 from various environmental samples. The sensitivity of the developed method is 100000 times higher than the conventional method, which can quantify coenzyme F430 at 0.1 femto mol. The mothod made it possible to quantify coenzyme F430 from marine sediments, microbial mats, paddy soils, groundwater etc. Coenzyme F430 was detected coalbeds located at 2000 m below seafloor offshore Shimokita Peninsula (world record of deep biosphere), suggested presence of active methanogens. This study was published from Science.
The scientific results was published from totally 4 peer reviewed scientific journals and presented at 11 conferences and received high international evaluation.

Free Research Field

有機地球化学