Development of bienzyme immobilized electrode by using interstrand cross-linked DNAs.

2014 Fiscal Year Final Research Report

• Project/Area Number: 25620121
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Analytical chemistry
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: KOMATSU Yasuo 独立行政法人産業技術総合研究所, 生物プロセス研究部門, 研究グループ長 (30271670)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: バイオセンサー / DNA / 電気化学 / グルコース / オリゴヌクレオチド / 酵素

Outline of Final Research Achievements

Glucose concentrations can be determined by using the coupled reaction system of glucose oxidase (GOx) and horseradish peroxidase (HRP). We integrated both GOx and HRP on an electrode surface through attachment at the termini of double-stranded oligodeoxynucleotides (ODNs). Reduction currents based on the coupled reactions were significantly increased on the electrode which tethered both enzymes by an interstrand crosslinked (ICL)-ODN. The current values were largely affected by the relative positions of enzymes on ICL-ODNs, and the detection sensitivity became the highest when GOx and HRP were distal and proximal to the electrode surface, respectively. The bienzyme/ICL-ODN complexes could also be constructed on a microelectrode surface and could also sensitively detect glucose concentrations. Here we report the construction of an electrode carrying bienzyme/ICL-ODN complex and the importance of enzyme binding positions on double stranded ODNs.

Free Research Field

核酸化学