2014 Fiscal Year Final Research Report
New miRNA detecting system utilizing fluorescent DNA triplexes and rolling circle amplification
| Project/Area Number |
25620126
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bio-related chemistry
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
SEIO KOHJI 東京工業大学, 生命理工学研究科, 准教授 (20313356)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | 蛍光核酸 / microRNA |
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| Outline of Final Research Achievements |
We planed to develop new microRNA detection techniques using fluorescent oligonucleotides and rolling circle amplification. As the fluorescent nucleosides developed 5-(3-methylbenzofuran-2-yl)deoxyuridine (dUMBF), the dUMBF derivatives incorporating various substituents on its benzofuran ring, and 7-deazaguanine incorporating benzofuranyl group at the 7 position. The fluorescent properties of these nucleosides and oligonucleotides incorporating them were studied.
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| Free Research Field |
核酸化学
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