Construction of a porphyrin capsule applicable to a photodynamic therapy

2014 Fiscal Year Final Research Report

• Project/Area Number: 25620130
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Bio-related chemistry
• Research Institution: Nagoya University
• Principal Investigator: NAKAJIMA Hiroshi 名古屋大学, 理学(系)研究科(研究院), 准教授 (00283151)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: チトクロムc / ポルフィリン / 光力学線療法 / 一重項酸素発生

Outline of Final Research Achievements

Cytochrome c552 is a thermostable protein deriving from a thermophile. We have successfully prepared a stable composite of a cytochrome c552 mutant in an apo-form with protoporphyrin IX. While protoporphyrin IX is insoluble to water at the neutral pH, the composite showed high solubility to water and high activity as a photosensitizer. The composite produced single oxygen by photo-irradiation for more than 1 hour by a quantum yield of 19% in water. The obtained yield was rather lower than methylene blue (MB), a commercially available photosensitizer, exhibiting the highest quantum yield in the single oxygen production in aqueous solution (52%), while the composite is superior to MB in the functional durability. Under the same experimental conditions, the photosensitizing activity of MB was deprived in 20min and was overtaken by the composite at the 1hr-irradiation. The result indicates that the composite is a potential photosensitizer for the single oxygen production in water

Free Research Field

生体機能関連化学