Development of RNA-diagnosis system for the analysisi of RNA and RNA-protein complexes

2014 Fiscal Year Final Research Report

• Project/Area Number: 25620132
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Bio-related chemistry
• Research Institution: Kyoto Institute of Technology
• Principal Investigator: MURAKAMI Akira 京都工芸繊維大学, 工芸科学研究科, 教授 (60210001)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: RNA診断 / 非コードRNA / miRNA / RISC / ピレンプローブ / 均一系蛍光検出

Outline of Final Research Achievements

Human genome project has revealed that the mechanism of gene expression is much more complicated than that we have believed to date. This project focused on the novel RNA-diagnosis system for understanding the detailed mechanism of gene expression where RNAs play crucial roles. As results, we innovated the following systems: (1)Fluorescent-RNA-detection system to detect specific RNA in homogeneous physiological condition. The system was successfully applied to detect unusual splicing products of the muscular dystrophy. (2)Fluorescent-RNA-detection system to detect RNA-protein complexes such as telomerses and RNA-induced silencing complex (RISC). The system successfully detected telomerase in fixed cells and RISC in living cells. The system can be applied to the development of the novel curing methods of various diseases such as cancers.

Free Research Field

核酸化学、RNA診断、遺伝子制御法開発