2014 Fiscal Year Final Research Report
Determination of molecular mechanism of loop-specific structural change and inhibitory effect for Dicer mediated reaction
| Project/Area Number |
25620134
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bio-related chemistry
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| Research Institution | Osaka University |
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Principal Investigator |
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| Research Collaborator |
FUKUZUMI Takeo 大阪大学, 産業科学研究所, 特任助教 (00592768)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | マイクロRNA / 結合分子 / ループ / 構造変化 / Dicer / 阻害 |
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| Outline of Final Research Achievements |
We hypothesized that conjugation of a hydrogen bonding module to the coumarin chromophore would be effective to modulate the electronic spectra upon binding to RNA, and may provide useful molecular indicators for structure-activity studies on small molecule-RNA interaction. the coumarin-naphthyridine conjugate TT7 showed marked absorption shift upon binding to the secondary structure of RNA. TT7-binding to the int-loop RNA was a good candidate of further study on the ligand-bound structure due to a clear 1:1 binding stoichiometry, a high affinity, and competitive binding to rev peptide. In addition, TT7 can be a potentially useful fluorescence indicator for FID assay of ligand binding to RNA of interests.
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| Free Research Field |
ケミカルバイオロジー
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